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Tobacco budworm P-glycoprotein: biochemical characterization and its involvement in pesticide resistance

机译:烟草芽虫P-糖蛋白的生化特性及其与农药抗性的关系

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摘要

Since pesticides have been shown to interact with P-glycoprotein (P-gp), the purpose of this study was to examine the possible role of P-gp in pesticide resistance in the tobacco budworm (Heliothis virescens). Using three P-gp antibodies, P-gp expression in various resistant populations of tobacco budworms was found to be 2-6-times that of the susceptible larvae. Tobacco budworm P-gp was glycosylated and localized primarily in the cuticle and fat body with little expression in the mid gut. To determine the role of P-gp in pesticide resistance, resistant tobacco budworm larvae were treated with a P-gp inhibitor, quinidine, and challenged with various doses of ihiodiearb. Inhibition of P-gp decreased the LD5() for thiodicarb by a factor of 12.5. Quinidine treatment did not result in a significant inhibition of the /M50 system nor did it alter the feeding of the larvae, suggesting the potential involvement of P-gp in pesticide resistance. An age-dependent inci'ease in P-gp expression was detected in resistant larvae as compared to control, susceptible larvae. This correlates with the reported age-dependent increase in resistance and is further evidence supporting the role of P-gp in the development of pesticide resistance.
机译:由于已证明农药与P-糖蛋白(P-gp)相互作用,因此本研究的目的是检验P-gp在烟草芽虫(Heliothis virescens)中对农药抗性的可能作用。使用三种P-gp抗体,发现烟草抗性虫的各种抗性种群中P-gp的表达是易感幼虫的2-6倍。烟草芽虫P-gp被糖基化并主要定位在表皮和脂肪体内,在肠道中部很少表达。为了确定P-gp在抗药性中的作用,用P-gp抑制剂奎尼丁处理抗药性烟草芽虫幼虫,并用各种剂量的ihiodiearb攻击。 P-gp的抑制使硫双威的LD5()降低12.5倍。奎尼丁处理未导致对/ M50系统的显着抑制,也未改变幼虫的摄食,表明P-gp可能与杀虫剂抗性有关。与对照易感幼虫相比,在抗性幼虫中检测到了P-gp表达的年龄依赖性增强酶。这与所报道的抗药性的年龄依赖性增加相关,并且进一步证明了P-gp在农药抗药性发展中的作用。

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